Day 33-The Mannitol PR Broth results were - on the Gram positive bacteria,which made my + Bacteria: Bacillus Cereus, and it also was negative on my Citrate test, which resulted in E.Coli on my Gram - test.The EMB test turned out metallic green,which meant my E.coli results were correct

EMB test

Citrate Test

Mannitol PR Broth

Day 32-Ran a EMB test on the Gram - Bacteria to see if I had E.coli.Incubated test for 24hrs.

Day 31-Ran the VP test on both Bacterias and waited a 1hr for the results,VP was negative on Gram - Bacteria and positive on Gram + Bacteria,Did the Mannitol Pr Broth test on Gram + and incubated for 48hrs.Ran the Citrate test on the Gram - Bacteria,incubated for 48hrs.

VP Test for Gram -

VP Test for Gram +

Day 30-Still waiting on the VP results

Day 29-On the gram + the starch test was + and on the gram -,the lactose pr broth was was +,the next test on both bacterias was the VP test,incubated the VP for 48 hrs on both tests

Starch Test

Lactose PR Broth for Gram -

Day 28-The Hemolysis test results were + on the Gram positive test

Hemolysis Test

Day 27-On the Gram +,I did a Hemolysis and starch test and incubated the hemolysis for 24 hrs and the starch for 48 hrs.The TSI test was negative for H2s,the next test was MacConkey which it had growth,next test was a Oxidase on Gram - and the results were negative.On Gram neg, the next test was Lactose Broth,incubated for 48 hrs.

Oxidase Test

TSI(H2S)

Day 26-Of Glucose results were a fermenter on the Gram - bacteria,Ran a TSI test and incubated for 24 hrs.On the Gram +,On gram +,I ran a Catalase test,it was + as well.

Catalase Test

Of Glucose

Day 25-Started my Of Glucose test for Gram Negative Bacteria,incubated for 24 hrs.Ran a Spores test on my Gram +,spores were presented

Endospores

Day 24-Separated my Gram Positive Bacteria,The results were Bacilli and separated my Gram Negative

Gram Negative

Gram Positive

Day 23-Started Unknowns with new broth,2 TSA 5% sheep blood agars,1 PEA,1MAC,incubating for 24 hrs.

PEA

5%BLOOD AGAR

MACCONKEY

5%BLOOD AGAR

Day 22-Of Glucose test results were oxidizer on the GN bacteria.Started the MacCponkey test and incubating for 24 hrs.Hemolysis test was + on the GP Bacteria.Incubating Starch test for 48 hrs.

OF Glucose

Hemolysis

Day 21-Endospores test results were + on gram positive test.Catalase test was + on gram positive test,Incubated a Hemolysis test for 24hrs.and incubating a starch test for 48hrs.

Day 20-Started the Of Glucose test for Gram Neg.incubating the test for 24-48hrs.Made new TSA slants incubated them for 24hrs

Day 19-Completed Gram stain and Isolated slants.Have indentified the Gram negative and Gram positive.

Day 18-Picked isolated colony from the TSA and made 2 slants and 2 Gram stains

Day 17-Started Unknowns,Streaked 2 TSA,1MAC,1PEA plates,Gram stain the Unknown

Day 16-Started PEA,CNA,Mannitol Salt,MacConkley,EMB,Blood Agar Tests

Mannitol Salt

EMB

Blood Agar

MAC

                                                                              PEA

CNA

Day 15-Lab Practical

Day 14-Completed the MRVP,Citrate,Tyrpt Tests

Trypt

MR Test

Citrate

Day 13-Oxidation-fermentation,Phenol Red Broth,Methyl Red and Voges-Proskauer,Tyrpt,TSI Tests

Day12-Catalase,Oxidase,Nitrate,Starch,Urea Hydrolysis,Gelatin,Coagulase,TSI tests

                                                               Urease Test

Day 11-Started Streak Plates